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MOLECULAR BIOLOGY (Principles and Practices) introduces undergraduates, postgraduates as well as research students of biotechnology to basic experimental molecular biology laboratory techniques. Emphasis has been given to techniques encountered in practical classes with the principles and theories behind this explained in detail. No contemporary book on Practical Molecular Biology would be complete without chapters on Bio-Safety, Laboratory Set-up, Molecular Biology Calculations and Glossary. These topics have been extensively covered in this book. This is essential reading for all biosciences students, biotechnology and preclinical medical students for whom practical molecular biology forms part of the syllabus.
Table Of Contents :
UNIT I : MOLECULAR BIOLOGY LABORATORY-AN INTRODUCTIONPart 1 - General Laboratory FacilitiesPart 2 - General Laboratory PracticesPart 3 - Laboratory Safety Guidelines and Health HazardsUNIT II : CALCULATIONS USED IN MOLECULAR BIOLOGYPart 1 - Calculations used in Molecular BiologyUNIT III : DNA ISOLATION AND PURIFICATIONPart 1 - Plasmid DNAPart 2 - Bacterial (E.Coli) DNAPart 3 - Plant Genomic DNAPart 4 - Animal Genomic DNAUNIT IV : ELECTROPHORESISPart 1 - A General AccountPart 2 - Agarose Gel Electrophoresis of DNA Part 3 - Polyacrylamide Gel Electrophoresis of DNAPart 4 - Polyacrylamide Gel Electrophoresis of ProteinsUNIT V : CHECKING QUALITY AND QUANTITY OF DNAPart 1 - Spectrophotometric MethodPart 2 - Electrophoretic MethodUNIT VI : RESTRICATION ENDONUCLEASESPart 1 - A General AccountPart 2 - Restriction Enzyme Digestion and Size Determination of DNA Fragments.Part 3 - Restriction Mapping of Lambda DNA with three Enzymes viz Eco RI, Bam HI and Hind IIIUNIT VII : LIGATIONPart 1 - A General AccountPart 2 - Ligation of a DNA Fragment, Carrying Gene of Interest, to a Plasmid for Constructing a Recombinant DNA MoleculeUNIT VIII : TRANSFORMATIONPart 1 - Transformation a General AccountPart 2 - Bacterial Plasmid TransformationPart 3 - Agrobacterium-Mediated Transformation in Plants(e.g., Nicotiana Tabacum)UNIT IX : POLYMERASE CHAIN REACTIONPart 1 - PCR-a General AccountPart 2 - Performing Amplification of DNA using PCR in a Routine Laboratory ExperimentUNIT X : MOLECULAR MARKERSPart 1 - Molecular Markers-a General AccountPart 2 - RFLP (Restriction Fragment Length Polymorphism)Part 3 - RAPD (Random amplified polymorphic DNA)Part 4 - AFLP (Amplified Fragment Length Polymorphism)Part 5 - Simple Sequence Repeats (SSRs) or Micro SatellitesUNIT XI : MOLECULAR MARKERS AND MAPPINGPart 1 - Molecular Markers and MappingUNIT XII : DNA SEQUENCINGPart 1 - A General AccountPart 2 - Performing DNA Sequencing in Laboratory by the Sanger`s Dideoxy Method using Sequenase EnzymeUNIT XIII : IN VITRO MUTAGENESISPart 1 - A General AccountPart 2 - Performing Oligonucleotide Directed Mutagenesis of Single Stranded DNAMOLECULAR BIOLOGY GLOSSARYSOURCES AND SUGGESTED READINGIMPORTANT WEBSITESANNEXURE I : Preparation of Cleansing solution, Acid and Albati (1 N) solution, Standard buffer solutionsANNEXURE Il : Molecular Biology Laboratory Waste DisposalANNEXURE IlI : Hazard Symbols and PrecautionsANNEXURE IV : Inactivation of Ethidium BromideANNEXURE V : Biological Agents Causing BiohazardsANNEXURE Vl : Risk GroupsANNEXURE VIl : Biosafety Committees in IndiaANNEXURE VIIl : Preparation of Buffer Equilibrated PhenolANNEXURE IX : Miscellaneous InformationANNEXURE X : Periodic Table of the Elements ISBN
9788131807446
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Pages : 350
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