This work outlines the latest information in microbial genetics in a lucid manner. The genetics of bacteria, and recombinant DNA technology and its applications are comprehensively discussed in a clear and concise manner. Part I: Microbial Genetics Basic molecular genetics: DNA structure, replication and repair Basic molecular genetics: Gene expression and regulation Methods of genetic analysis Conjugation Transformation Transduction Techniques for studying bacteriophages Plasmids Genetic rearrangements and their evolutionary significance Part II: Recombinant DNA Technology Recombinant DNA Technology Construction of DNA libraries Proteins expression in bacteria Sequencing of DNA and proteins Synthesis of oligonucleotides Site-specific mutagenesis Protein engineering Gene isolation and synthesis Polymerase Chain Reaction (PCR) DNA Fingerprinting Random Amplification of Polymorphic DNA (RAPD) Restriction mapping Application of rDNA technology in agriculture, health, medicine and industry Containment biological and physicalISBN - 9788179933237